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streptavidin conjugated horseradish peroxidase  (Vector Laboratories)


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    Structured Review

    Vector Laboratories streptavidin conjugated horseradish peroxidase
    Streptavidin Conjugated Horseradish Peroxidase, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/SA-5594-1/DyLight+594+Streptavidin/pmc13133793-44-84-89
    Average 93 stars, based on 100 article reviews
    streptavidin conjugated horseradish peroxidase - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Nasal trigeminal projections and medullary dorsal horn neuronal activation during voluntary diving in rats
    Article Snippet: .. The tissue was then incubated for 2 h with fluorescent Streptavidin Dylight (Vector SA-5594; red, AF 594). .. The tissue free-floating in PBS was rearranged into serial order, mounted on slides, and then coverslipped using hard set mounting media (Vector H-1700).

    Article Title: Study on the Role of Short Peptide LCKLSL Targeting ANXA2 in Retinal Neovascularization
    Article Snippet: .. The isolated retina was treated with 5% bovine serum albumin (BSA) containing 0.1% Triton-100 at room temperature for 90 min. After removing the blocking solution, the retina was washed three times with PBS (pH 7.4) for 10 min each, then transferred to a working solution of the vascular-specific marker isolectin B4 (IB4; B1205; 1:200; Vector Laboratories, Burlingame, CA, USA) and incubated at 4°C in the dark for 12 h. After washing three times with PBS for 10 min each, the retina was incubated with streptavidin-conjugated horseradish peroxidase (SA-5594; 1:500; Vector Laboratories) at room temperature in the dark for 2 h, followed by three 10-min PBS washes. .. It was then transferred to a diluted working solution of ANXA2 primary antibody (11256-1-AP; 1:400; Proteintech Group, Rosemont, IL, USA) and incubated at 4°C in the dark for 12 h. After removing the primary antibody and washing three times with PBS for 10 min each, the retina was incubated with goat anti-rabbit secondary antibody (A-11008; 1:500; Thermo Fisher Scientific, Waltham, MA, USA) at room temperature in the dark for 2 h. After final PBS washes, the retina was carefully flattened on a slide under a stereomicroscope, mounted with anti-fluorescence quenching mounting medium, and covered with a coverslip.

    Article Title: LncRNA DANCR Activates p38/ mTOR ‐Mediated Autophagy via ANXA2 to Exacerbate Oxygen‐Induced Retinal Neovascularization in Mice
    Article Snippet: .. The retinal tissues were then incubated overnight at 4°C with a biotinylated Griffonia Simplicifolia Lectin I Isolectin B4 (IB4) (1:100, Vector Laboratories B1205, USA, RRID: AB_2314661), followed by treatment with streptavidin DyLight 594 (1:100, Vector Laboratories SA‐5594‐1, RRID: AB_2336418), or IBA1 antibody (1:400, Wako 019‐19 741, RRID: AB_839504), followed by treatment with an Alexa Fluor 488 goat anti‐Rabbit IgG antibody (1:300, Invitrogen A11008, RRID: AB_143165). .. After mounting the retina flat, immunofluorescence images were captured using an LSM880 confocal microscope (Carl Zeiss, Germany).

    Article Title: Cytotoxic activity of pyrimethanil on human testicular teratocarcinoma NT2/D1 cells
    Article Snippet: Upon overnight incubation, appropriate secondary antibodies, biotinylated goat anti-rabbit IgG (BA-1000) or horse anti-mouse IgG (BA-2000) (Vector Laboratories, Burlingame, CA, U. S.) diluted 1:250 in 1 % BSA and PBS and 0.1 % Triton X-100 were applied for 1 h at RT. .. For fluorescence detection, cells were incubated for 1 h at RT in DyLight 488 Streptavidin (SA-5488) (Vector Laboratories, Burlingame, CA, U. S.) or DyLight 594 Streptavidin (SA-5594) (Vector Laboratories, Burlingame, CA, U. S.) diluted 1:500 in PBS. .. Staining of actin filaments (F-actin) was achieved by incubating cells for 1 h at RT in CytoPainter Phalloidin-iFluor 594 Reagent (ab176757, Abcam), diluted 1:3000 in PBS.

    Isolation:

    Article Title: Study on the Role of Short Peptide LCKLSL Targeting ANXA2 in Retinal Neovascularization
    Article Snippet: .. The isolated retina was treated with 5% bovine serum albumin (BSA) containing 0.1% Triton-100 at room temperature for 90 min. After removing the blocking solution, the retina was washed three times with PBS (pH 7.4) for 10 min each, then transferred to a working solution of the vascular-specific marker isolectin B4 (IB4; B1205; 1:200; Vector Laboratories, Burlingame, CA, USA) and incubated at 4°C in the dark for 12 h. After washing three times with PBS for 10 min each, the retina was incubated with streptavidin-conjugated horseradish peroxidase (SA-5594; 1:500; Vector Laboratories) at room temperature in the dark for 2 h, followed by three 10-min PBS washes. .. It was then transferred to a diluted working solution of ANXA2 primary antibody (11256-1-AP; 1:400; Proteintech Group, Rosemont, IL, USA) and incubated at 4°C in the dark for 12 h. After removing the primary antibody and washing three times with PBS for 10 min each, the retina was incubated with goat anti-rabbit secondary antibody (A-11008; 1:500; Thermo Fisher Scientific, Waltham, MA, USA) at room temperature in the dark for 2 h. After final PBS washes, the retina was carefully flattened on a slide under a stereomicroscope, mounted with anti-fluorescence quenching mounting medium, and covered with a coverslip.

    Blocking Assay:

    Article Title: Study on the Role of Short Peptide LCKLSL Targeting ANXA2 in Retinal Neovascularization
    Article Snippet: .. The isolated retina was treated with 5% bovine serum albumin (BSA) containing 0.1% Triton-100 at room temperature for 90 min. After removing the blocking solution, the retina was washed three times with PBS (pH 7.4) for 10 min each, then transferred to a working solution of the vascular-specific marker isolectin B4 (IB4; B1205; 1:200; Vector Laboratories, Burlingame, CA, USA) and incubated at 4°C in the dark for 12 h. After washing three times with PBS for 10 min each, the retina was incubated with streptavidin-conjugated horseradish peroxidase (SA-5594; 1:500; Vector Laboratories) at room temperature in the dark for 2 h, followed by three 10-min PBS washes. .. It was then transferred to a diluted working solution of ANXA2 primary antibody (11256-1-AP; 1:400; Proteintech Group, Rosemont, IL, USA) and incubated at 4°C in the dark for 12 h. After removing the primary antibody and washing three times with PBS for 10 min each, the retina was incubated with goat anti-rabbit secondary antibody (A-11008; 1:500; Thermo Fisher Scientific, Waltham, MA, USA) at room temperature in the dark for 2 h. After final PBS washes, the retina was carefully flattened on a slide under a stereomicroscope, mounted with anti-fluorescence quenching mounting medium, and covered with a coverslip.

    Marker:

    Article Title: Study on the Role of Short Peptide LCKLSL Targeting ANXA2 in Retinal Neovascularization
    Article Snippet: .. The isolated retina was treated with 5% bovine serum albumin (BSA) containing 0.1% Triton-100 at room temperature for 90 min. After removing the blocking solution, the retina was washed three times with PBS (pH 7.4) for 10 min each, then transferred to a working solution of the vascular-specific marker isolectin B4 (IB4; B1205; 1:200; Vector Laboratories, Burlingame, CA, USA) and incubated at 4°C in the dark for 12 h. After washing three times with PBS for 10 min each, the retina was incubated with streptavidin-conjugated horseradish peroxidase (SA-5594; 1:500; Vector Laboratories) at room temperature in the dark for 2 h, followed by three 10-min PBS washes. .. It was then transferred to a diluted working solution of ANXA2 primary antibody (11256-1-AP; 1:400; Proteintech Group, Rosemont, IL, USA) and incubated at 4°C in the dark for 12 h. After removing the primary antibody and washing three times with PBS for 10 min each, the retina was incubated with goat anti-rabbit secondary antibody (A-11008; 1:500; Thermo Fisher Scientific, Waltham, MA, USA) at room temperature in the dark for 2 h. After final PBS washes, the retina was carefully flattened on a slide under a stereomicroscope, mounted with anti-fluorescence quenching mounting medium, and covered with a coverslip.

    Fluorescence:

    Article Title: Cytotoxic activity of pyrimethanil on human testicular teratocarcinoma NT2/D1 cells
    Article Snippet: Upon overnight incubation, appropriate secondary antibodies, biotinylated goat anti-rabbit IgG (BA-1000) or horse anti-mouse IgG (BA-2000) (Vector Laboratories, Burlingame, CA, U. S.) diluted 1:250 in 1 % BSA and PBS and 0.1 % Triton X-100 were applied for 1 h at RT. .. For fluorescence detection, cells were incubated for 1 h at RT in DyLight 488 Streptavidin (SA-5488) (Vector Laboratories, Burlingame, CA, U. S.) or DyLight 594 Streptavidin (SA-5594) (Vector Laboratories, Burlingame, CA, U. S.) diluted 1:500 in PBS. .. Staining of actin filaments (F-actin) was achieved by incubating cells for 1 h at RT in CytoPainter Phalloidin-iFluor 594 Reagent (ab176757, Abcam), diluted 1:3000 in PBS.

    Immunofluorescence:

    Article Title: Collectin-11 regulates osteoclastogenesis and bone maintenance via a complement-dependent mechanism.
    Article Snippet: A biotinylated secondary antibody was applied for 1 h at RT, followed by ABC kit and DAB (Vector Labs), counterstaining with hematoxylin, and dehydration for mounting with DPX (Sigma). .. For immunofluorescence, a biotinylated anti- rabbit secondary antibody (Vector Labs; BA- 1000) was applied for 1 h at RT, followed by FITC- conjugated streptavidin (GeneTex; GTX30950) or streptavidin- 594 (Vector Labs; SA- 5594). .. Nuclear staining was performed using DAPI (1:10,000 in PBS; Life Technologies) before mounting with PermaFluor (LabVision).

    Article Title: Collectin-11 regulates osteoclastogenesis and bone maintenance via a complement-dependent mechanism
    Article Snippet: A biotinylated secondary antibody was applied for 1 h at RT, followed by ABC kit and DAB (Vector Labs), counterstaining with hematoxylin, and dehydration for mounting with DPX (Sigma). .. For immunofluorescence, a biotinylated anti-rabbit secondary antibody (Vector Labs; BA-1000) was applied for 1 h at RT, followed by FITC-conjugated streptavidin (GeneTex; GTX30950) or streptavidin-594 (Vector Labs; SA-5594). .. Nuclear staining was performed using DAPI (1:10,000 in PBS; Life Technologies) before mounting with PermaFluor (LabVision).



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